Why dcpip is blue

DCPIP becomes colorless as it is reduced by intercepting electrons from the electron transport chains in the thylakoid membranes. When the dye is oxidized, it is blue. When reduced, however, it turns colorless. Some of the earliest signs of deficiency include fatigue, red gums, easy bruising and bleeding, joint pain and rough, bumpy skin. As the deficiency progresses, bones may become brittle, nail and hair deformities can develop, wounds may take longer to heal and the immune system suffers.

Draw up some of the clear liquid into a pipette and then add it, one drop at a time , to a test tube containing a light blue solution of DCPIP. If the extract is acid the colour will change from blue to red. Continue to add more and see if the colour disappears altogether. A blood test can be taken to measure vitamin C levels and may help to confirm the diagnosis.

Your doctor may also suggest some other blood tests to check for other deficiencies in your diet. For example, vitamin C is also needed for the absorption of iron from food. The direct visual titration of vitamin C with indophenol cannot be used when the vitamin is to be assayed in materials that give deeply coloured extracts.

The colorimetric measurement of the large excess of the dye added to the sample-extract in these methods introduces an extra step of photoelectric colorime- try. Divide the total number of iodine drops by the number of drops per mg of vitamin C from the reference sample. Oxidation and reduction in terms of oxygen transfer Oxidation is the gain of oxygen. Reduction is the loss of oxygen. DCIP 2,6-dichlorophenolindophenol is a dye which is blue in its oxidized form and colorless in its reduced form.

In this experiment, as described by scienceprojectlab. You will measure how much of your iodine solution it takes to oxidize a known amount of vitamin C. You can then use your iodine titration solution to determine the amount of vitamin C from test samples of different orange juices. DCPIP can be used to measure the rate of photosynthesis. If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink in acid conditions, is reduced to a colorless compound by ascorbic acid.

The vitamin C content of food may be reduced by prolonged storage and by cooking because ascorbic acid is water soluble and is destroyed by heat [6,8]. Steaming or microwaving may lessen cooking losses. Fortunately, many of the best food sources of vitamin C, such as fruits and vegetables, are usually consumed raw.

DCPIP can be used to measure the rate of photosynthesis. The principle of this method is a titration with dichlorophenolindophenol or phenol-indodichlorophenol, also known as DCPIP. When exposed to light in a photosynthetic system, the dye is decolorised by chemical reduction. Fill the biurette with the standardized vitamin C solution ppm.

For colorimetry this should be diluted with water to give an absorbance between 1 and 1. Using ice cold phosphate buffer solution and ice cold sucrose solution is necessary to slow enzyme action and prevent damage to the chloroplasts before the experiment begins. There are no lysosomes in the chloroplast solution since the chloroplasts are isolated from the rest of the plant cell. The Hill reaction is formally defined as the photoreduction of an electron acceptor by the hydrogens of water, with the evolution of oxygen.

We can measure the rate of the Hill reaction in isolated chloroplasts. Follow this easy, step-by-step activity: Determine which food s you want to test for vitamin C. Put 15 drops of indophenol solution in a test tube for each food item you want to test. DCPIP is a dark blue solution that becomes lighter as it is reduced. It provides experimenters with a simple visual test and easily observable light reaction. In another approach to studying photosynthesis, light-absorbing pigments such as chlorophyll can be extracted from chloroplasts.

Hill Reaction of the Photosynthesis was found by Robert Hill He found that isolated chloroplasts from plants can discharge oxygen O2 when they are illuminated by sunlight or by a light source same to sunlight in the prescence of suitable electron acceptor such as Ferricyanide.

Why was an Isolation Medium used? To help seperate the organelles out. The isolation medium can also contain the isotonic solution which means that it prevents the cell bursting or shrinking due to osmotic gain or loss of water.

The source of the electrons is the water. In this investigation, DCPIP 2,6-dichlorophenol-indophenol , a blue dye, acts as an electron acceptor and becomes colourless when reduced, allowing any reducing agent produced by the chloroplasts to be detected.